![]() ![]() ![]() These tubes were labeled MR and set aside for a few minutes for observation. To one 4-species tube set, 4-5 drops of methyl red indicator were added and the test tube was shaken. The glucose phosphate broth cultures were removed from incubation half of each tube’s contents was transferred to a new test tube. The cultures were placed to the side for the moment (in the storage rack) to go through a 24-hour incubation period. These broth cultures would later be differentiated into eight MR-VP broths, which would then have MR and VP tests run on them using Barritts A and/or B reagent and methyl red indicator. Methyl Red and Voges-Proskauer Broth PreparationĪn inoculating loop was used to transfer an inoculum of each species in the Materials section to its own sterile glucose phosphate broth tube, with aseptic technique being strictly followed regarding tube and loop sterilization. These new culture deeps were then transferred to the storage rack with the rest of the cultures made previously in the lab to be incubated for 24 hours. Aseptic technique (such as flaming the inoculating needle at proper times) was maintained in all bacterial transferals. The four Simmons Indole Motility agar deeps were inoculated with a vertical stab from an inoculation needle, each with a different type of bacteria (as listed in the Materials section). The freshly-inoculated SCA and urease slants were placed to the side in the storage rack for 24 hours of incubation. However, SCA and urease slants were inoculated with a loop and were not stabbed in the butt. Aseptic technique pertaining to test tubes and inoculating instruments was maintained in all bacterial transfers. All slants were inoculated via inoculation loop in a zigzag pattern across the surface of the slant from the lower-elevation end to the higher-elevation end, like the streak configuration seen in the TSI slants. One Simmons Citrate Agar slant and one urease slant was reserved for each species of bacteria listed in the Materials section above. Simmons Citrate (SCA) and Urease Agar Slants Once this procedure had been applied to all slants, the tubes were placed in the storage rack for a 24-hour period incubation. The needle was not flamed between the stabbing and streaking of the agar. The TSI slant was first stabbed with the needle, before the needle was dragged in a zigzag pattern across the surface of the slant from the lower-elevation end to the higher-elevation end. Proper aseptic technique concerning inoculation and test tubes was maintained in all bacterial transferals. The four Triple Sugar Iron agar slants were each inoculated via inoculating needle with a different species of bacteria (those listed in Materials section). All three inoculated plates were then placed on a storage rack to be incubated for 24 hours. The genus species initials for each bacterial type were written down in the respective quadrant in which that species was located. The plates were labeled on the bottom with permanent marker, split into four quadrants for each streak. Aseptic technique was maintained in all bacterial transfers (ex: flaming the inoculating loop at proper times). Each plate had four resulting one-inch streaks, one in each quadrant and each made up of a different species inoculum. The McConkey, Eosin Methylene Blue (EMB), and Hektoen plates were each inoculated with a streak of every bacterial variety listed in the Materials section of this report. – tryptic soy agar plate cultures (pure) of: ![]() – 4 Sulfide-Indole Motility (SIM) agar deeps – 1 Eosin Methylene Blue (EMB) agar plate The materials needed for this lab were as follows: This was done to determine such characteristics as ability to ferment lactose/glucose/sucrose, produce hydrogen sulfide/acids/acetoin, and utilize urea or citrate. The objective of this lab was to run differential tests on Escherichia coli, Pseudomonas fluorescens, Bacillus subtilis, and Proteus vulgaris. Lab 4: Biochemical Activities of Bacteria I I. Lab 7: Isolation of Microorganisms From Soil Lab 6: Biochemical Activities of Bacteria II, Unknown Identification, and Lab 3: Negative, Acid Fast, Endospore, and Capsule Staining Lab 2: Gram Staining and Using the Spectrophotometer Lab 1: Inoculations, Smear Preps, and Simple Staining ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |